Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions

The relationship between hydrogen peroxide and polyamine metabolism the role of NADPH oxidase (NOX) in this under drought conditions has not yet been clarified. To reveal relationship, expression levels genes metabolism, such as arginine decarboxylase (ADC), agmatine aminohydrolase (AIH), spermidine synthase (SPDS), S-adenosyl methionine (SAMDC), diamine (DAO) (PAO) were determined by RT PCR stress combined with exogenous (H2O2) diphenyleneiodonium chloride (DPI) treatments maize seedlings. Also, some basic parameters (leaf water potential, lipid peroxidation), (putrescine, spermidine, spermine), gene NOX measured stress. Exogenous H2O2 induced contents up-regulating polyamine-synthesizing downregulating oxidizing genes. Moreover, when enzyme was inhibited DPI, metabolic pathway tended toward degradation rather than production. regulated to promote their synthesis, had a key regulation.